Macular Degeneration: Bopp S

Bopp S. · No affiliation provided · Br J Ophthalmol. · Pubmed #17895411 No free full text.

This publication has no abstract.

Tatar O, Adam A, Shinoda K, Yoeruek E, Szurman P, Bopp S, Eckardt C, Bartz-Schmidt KU, Grisanti S. · University Eye Hospital at the Center for Ophthalmology, Eberhard-Karls University Tuebingen, Germany. · Retina. · Pubmed #17621180 No free full text.

Abstract: PURPOSE: To examine the short- and long-term consequences of verteporfin photodynamic therapy (PDT) on inflammation with regard to infiltration of macrophages and leukocytes and expression of thy-1 in human choroidal neovascularization membranes (CNV) secondary to age-related macular degeneration (AMD). METHODS: Retrospective review of an interventional case series of 43 patients who underwent removal of CNV. Twenty patients were treated with PDT 3 to 246 days preoperatively. Twenty-three CNV without previous treatment were used as control. CNV were stained for CD34, CD105, cytokeratin 18, Ki-67, thy-1, an endothelial cell glycoprotein known to be upregulated only by inflammatory cytokines, CD68 (macrophages), and CD45 (common leukocyte antigen). RESULTS: Specimens treated by PDT 3 days previously showed significantly reduced endothelial thy-1 expression (P = 0.008), leukocyte (P=0.04) and macrophage (P=0.0063) infiltration, and proliferative activity (P=0.02) compared to control CNV. Specimens at longer intervals after PDT, in contrast, disclosed a significantly increased expression of thy-1 (P=0.004), infiltration with leukocytes (P=0.044) and macrophages (P=0.01), and proliferative activity (P=0.03) compared to CNV excised 3 days after PDT. CONCLUSIONS: The rebound effect after PDT seems to be based on an inflammatory response that contributes to enhanced proliferation. These data support the need for an anti-inflammatory therapy as adjuvant to PDT.

Petermeier K, Tatar O, Inhoffen W, Völker M, Lafaut BA, Henke-Fahle S, Gelisken F, Ziemssen F, Bopp S, Bartz-Schmidt KU, Grisanti S. · Univerisity Eye Clinic at the Centre for Ophthalmology, Eberhard-Karls University Tuebingen, Schleichstrasse 12-16, 72076 Tuebingen, Germany. · Br J Ophthalmol. · Pubmed #16613924 links to  free full text

Abstract: AIM: To evaluate the impact of verteporfin photodynamic therapy (PDT) on the induction of apoptosis in choroidal neovascular membranes (CNV) secondary to age related macular degeneration. METHODS: Retrospective review of 22 surgically excised CNV. 12 of these patients had been treated with PDT 3-146 days previously. Apoptotic cells were detected with the TUNEL technique and compared to the expression of CD34 (endothelial cells, EC), CD105 (activated endothelial cells), Ki-67 (proliferation marker), and cytokeratin18 (retinal pigment epithelial cells, RPE). RESULTS: CNV excised 3 days after PDT were characterised both by collapsed and patent vessels. The EC displayed a statistical significant positive TUNEL reaction when compared to the remaining treated CNV (p < 0.001) and untreated CNV (P = 0.002). The proliferative activity was reduced. CNV excised 1-5 months after PDT displayed a patent vascularisation and high proliferative activity. All membranes either treated or untreated disclosed only sporadic TUNEL positive cells within the stroma and the RPE. CONCLUSIONS: Verteporfin PDT leads to selective and effective damage of EC within CNV. Both patent and occluded vessels were lined by apoptotic EC. This finding and the increased expression of proliferation marker at later time points suggest that revascularisation after PDT is caused by angiogenesis rather than recanalisation.

Bopp S, Lucke K. · Tagesklinik Universitaetsallee, Department of Ophthalmology, Bremen, Germany. · J Cataract Refract Surg. · Pubmed #12663032 No free full text.

Abstract: A 72-year-old man presented with visual deterioration and cystoid macular edema (CME) 9 months after cataract surgery elsewhere. A slitlamp examination showed a large posterior capsule defect and a slightly decentered intraocular lens (IOL). In addition, a curved poly(methyl methacrylate) (PMMA) haptic-like structure was seen behind the IOL in the superoanterior vitreous cavity. The foreign body was entangled by vitreous fibers and moved in accordance with ocular motility. A broken PMMA haptic was suspected. During vitrectomy for removal of the suspected IOL haptic, an intact, posteriorly dislocated capsular tension ring (CTR) was found. It was cut in 2 and excised carefully via the sclerectomies. Postoperatively, the CME resolved and vision improved.